Hamza has an Achilles problem, which forced him to miss the Whites' league game with Al-Gaish last week.
However, the team were boosted by the news that Amr Zaki has recovered from a knee injury and is set to lead the frontline.
Zamalek, who have a 2-0 advantage following their first leg win in Cairo, will fly to Ivory Coast on Wednesday for the return leg next week.
Confident
Assistant coach Mohamed Helmi reflected the positive atmosphere of the team, saying that confidence is high at the White camp.
"Everyone is confident that we can make it to the last 16," he told FilGoal.com on Tuesday.
"The players are persistent to keep on the recent good work."
Zamalek jumped to second place in the Egyptian League after impressively beating Al-Gaish 2-1, despite being a goal down.
Zamalek Squad
Goalkeepers: Mohamed Abdul-Monsef, Abdul-Wahed Al-Sayed, Emad Al-Sayed.
Defenders: Karim Zekri, Beshir Al-Tabie, Mahmoud Fathallah, Amr Safti, Ahmed Magdi, Ahmed Ghanem, Tarek Al-Sayed, Mohamed Abdullah.
Midfielders: Mohamed Aboul-Ella, Hazem Emam, Mohamed Ibrahim, Mahmoud Abdul-Razeq 'Shikabala', Ahmed Abdul-Raouf.
Strikers: Amr Zaki, Mostafa Gaafar, Abdul-Halim Ali, Sherif Ashraf