The Whites are aiming to get off the bottom of Group A, following a first day defeat by Ahli, who also surpassed their bitter rivals in the Egyptian Super Cup.
However, a win could make up for Reiner Hollman's disappointing start with the Zamalek, who are yet to bear the fruits of some impressive summer signing.
Record signing Junior Agogo will miss the game through injury though, but Gamal Hamza will return to the frontline after serving a domestic suspension in last week's Super Cup defeat.
Full-back Ahmed Ghanem is suspended, while first-choice goalkeeper Abdul-Wahed Al-Sayed, who has an ankle problem, is likely to be replaced by Mohamed Abdul-Monsef.
New boys Alaa Kamal, Mahmoud Samir and Brazilian winger Ricardo are not registered in the African squad and will have to wait for their continental debut.
Vice-captain Mohamed Aboul-Ella should retain his place beside Ayman Abdul-Aziz in midfield.
On the other hand, Dynamos will be without key midfielder Desmond Maringwa, who is still nursing a groin injury.
Top scorer Edward Sadomba will be the main man for the visitors, following his brace in the 2-1 win over ASEC.
Dynamos are arguably the competition's surprise package this season after they ousted holders Etoile Sahel, beating the Tunisians giants twice in the process.