Massimo Ambrosini, 42, canceled Marco Di Vaio’s opener, 18, but Francesco Valiani, 79, handed the newcomers a perfect start to the season.
Coach Carlo Ancelotti lined up three new signings among his starting eleven as Ronaldinho, Mathieu Flamini and Gianluca Zambrotta made their debut for the Rossoneri.
The hosts were stunned by an early opener from Marco Di Vaio but Ronaldinho delivered a precise cross to Ambrosini who leveled the score just before the break.
The former Barcelona ace sent Filippo Inzaghi clear on goal, but the latter failed to control the ball and wasted a golden opportunity to give his side the lead.
Ukrainian striker Andriy Shevchenko came into action in the second half but couldn’t help his side score any goals.
Eleven minutes from time, Valiani sent a rocketing shot in the top corner to hand Bologna their first points of the season.