The veteran forward, who finished top scorer in the Serie A last season, is having another bright campaign with the Old Lady so far this term.
But King Alex's performances are yet to convince manager Marcelo Lippi, who selected Juve's third-choice striker Vincenzo Iaquinta for Tuesday's encounter.
A handful of players, including Gianluigi Buffon, Andrea Pirlo and Gianluca Zambrotta return to the Azzurri after missing last November's 1-1 draw in Greece.
Italy Squad:
Goalkeepers: Gianluigi Buffon (Juventus), Marco Amelia (Palermo), Morgan De Sanctis (Galatasaray)
Defenders: Daniele Bonera (AC Milan), Fabio Cannavaro (Real Madrid), Andrea Dossena (Liverpool), Alessandro Gamberini (Fiorentina), Fabio Grosso (Olympique Lyon), Nicola Legrottaglie (Juventus), Gianluca Zambrotta (Milan)
Midfielders: Alberto Aquilani (AS Roma), Mauro Camoranesi (Juventus), Daniele De Rossi (Roma), Riccardo Montolivo (Fiorentina), Simone Pepe (Udinese), Simone Perrotta (Roma), Andrea Pirlo (Milan)
Forwards: Antonio Di Natale (Udinese), Alberto Gilardino (Fiorentina), Vincenzo Iaquinta (Juventus), Fabio Quagliarella (Udinese), Giuseppe Rossi (Villarreal), Luca Toni (Bayern Munich)