Ahli's official site said that the 30-year-old twisted his ankle in Friday's session, which forced him to leave the training ground.
Abdul-Hamid's injury intensifies Ramzi Saleh's chances of starting in goal for the Egyptian champions for the second time running.
The Palestinian made his league debut against Ismaili in the recent 1-0 defeat last Thursday, following Abdul-Hamid's poor display against Petrojet a few days earlier.
Saleh was tipped by many as a better replacement for Essam Al-Hadari than the under-fire Abdul-Hamid, who helped the Red Devils win the CAF Champions League last year.
Ashour Back
Meanwhile, midielder Hossam Ashour is set to make his return against Asyut, following a knee injury.
The defensive midfielder missed Ahli's recent league games due to a medial ligament problem.
Ahli will also welcome back Ahmed Hassan and Ahmed Fathi, who missed the Ismaili game through suspension.