The International Football Association Board (IFAB) opted against implementing the new system, which was subject of intense discussions and debates during the past year.
"The door is closed. The decision was not to use technology at all," Jerome Valcke, general secretary of world governing body FIFA, told reporters.
The IFAB has taken a decision against the use of technology after watching presentations of two systems—Cairos, which uses a chip inserted in ball, and Hawkeye, currently used in tennis and cricket.
There were calls to use technology to get rid of costly refereeing mistakes, especially after France booked a place in the World Cup in controversial fashion.
Thierry Henry handed the ball in the build up to France's goal which earned Les Blues a berth in the football's most prestigious event at the expense of Ireland.
IFAB is made up of representatives of the four British football associations, while FIFA holds four votes.
Changes to the laws of the game must be approved by at least six votes.